Registro completo |
Provedor de dados: |
ArchiMer
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País: |
France
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Título: |
Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris
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Autores: |
Rolland, Jean-luc
Abdelouahab, Mahdia
Dupont, J.
Lefevre, F.
Bachere, Evelyne
Romestand, Bernard
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Data: |
2010-03
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Ano: |
2010
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Palavras-chave: |
Ls-Stylicin 1
Peptide
Shrimp
Antifungal
Agglutination
Vibrio
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Resumo: |
The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenoeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pl=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 mu M < MIC <2.5 mu M), a pathogenic fungus of shrimp, but lower antimicrobial activity against Gram () bacteria, Vibrio sp. (40 mu M < MIC <80 mu M). However, rLs-Stylicin1 is able to agglutinate Vibrio pennaeicidae in vitro in agreement with its potent LPS-binding activity on immobilized LPS of V. penaeicidae (dissociation constant (K-d) of 9.6 x 10(-8) M). This molecule with no evident homology to other hitherto described antimicrobial peptides but identified herein several species of penaeid shrimp is thought to be the first member of a shrimp antimicrobial peptide family, which we termed stylicins. (C) 2009 Elsevier Ltd. All rights reserved.
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Tipo: |
Text
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Idioma: |
Inglês
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Identificador: |
http://archimer.ifremer.fr/doc/00003/11387/8094.pdf
DOI:10.1016/j.molimm.2009.12.007
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Editor: |
Pergamon-elsevier Science Ltd
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Relação: |
http://archimer.ifremer.fr/doc/00003/11387/
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Formato: |
application/pdf
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Fonte: |
Molecular Immunology (0161-5890) (Pergamon-elsevier Science Ltd), 2010-03 , Vol. 47 , N. 6 , P. 1269-1277
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Direitos: |
2010 Elsevier Ltd All rights reserved.
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